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Omics Research // Epigenomics

Whole-Genome Bisulfite Sequencing

Sequence genome-wide methylation status quickly and accurately via US-based, massively parallel, next-generation sequencing (NGS).

What Is Whole-Genome Bisulfite Sequencing?

Whole-genome bisulfite sequencing (WGBS) is the most commonly used methodology of discovery efforts using epigenomics by measuring the methylation status of the nucleotide cystine across the entire genome.

WGBS combines sodium bisulfite conversion of the sequence with high throughput DNA sequencing. The sodium bisulfite reaction protects methylcytosines from conversion, whereas unmethylated cytosines are converted into uracil.

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Why Is Whole-Genome Bisulfite Sequencing Important?

The ability to characterize an entire organism’s methylation status offers possibilities in all fields of science. WGBS aids research in epigenome-wide studies by showing methylation status genome-wide. It can be used to identify gene expression regulation and transcription modification, which can identify different cell types and disease states.

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What Can You Do?
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Look Beyond the Genome

Go one step further from the gene sequence and study the transcriptional repression via methylation status

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Analyze CpG Sites

Discover gene silencing mechanisms by identifying CpG islands in promoter regions, and profile single nucleotide changes of CpG, CHG, and CHH

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Genome-Wide Studies

Study epigenetic mechanisms that regulate processes across the entire genome

On-Going Promotions

We're always adding new deals — check in often for updated promotions!

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WGBS Best Practices

Sample Preparation & DNA Extraction

DNA extraction from cells, tissue, FFPE, blood, and swab samples

Get your high-quality genomic DNA isolated from most sample types at Psomagen.

Sample Requirements

  • Sample input >200 ng and DIN >7.0
  • Library QC >12 nM at 330-350 bp

Library Preparation

Accel Methyl-Seq DNA library

Sequencing Instrumentation & Read Length

Illumina NovaSeq 6000 or HiSeqX

  • 2 x 151 bp read length

Bioinformatic Analysis

Basic analysis:

  • Data quality control
  • Mapping
  • Methylation profiling
  • Annotation

Advanced analysis:

  • Data quality control
  • Mapping
  • Methylation profiling
  • Annotation
  • Differentially methylated regions (CpGs)

Custom analysis also available.

Why Our Clients Choose Psomagen

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Superior Data Quality

Exceeds manufacturer benchmarks

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Fast Turnaround

Starting at one week for sequencing projects

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US-Based Processing

Sequencing and customer support in Rockville, MD

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Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system

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Automated Workflows

Increase scalability and improve reproducibility

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CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)

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Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

Quotes From Our Clients

I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.

Professor

University Research Laboratory

I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.

Professor

University Research Laboratory

I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.

Professor

University Research Laboratory

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!

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Request a Consultation

Create an account through our client portal and get in touch with your local field team to start designing a project.

We’ll help drive your research forward with the right tools for your research needs.

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Place an Order

After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.

Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.

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QC and Data Delivery

All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.

You can also see some project information and securely view all valid reports on the client portal site.

Customer Publications and Other Resources

A chromosome-level reference genome and pangenome for barn swallow population genomics
Whole genome sequencing identifies structural variants contributing to hematologic traits in the NHLBI TOPMed program
First complete mitochondrial genome of the Saharan striped polecat (Ictonyx libycus)
PSO Comprehensive!

What forms of whole-genome sequencing will get you the insights you need?