What Is Whole-Genome Bisulfite Sequencing?
Whole-genome bisulfite sequencing (WGBS) is the most commonly used methodology of discovery efforts using epigenomics by measuring the methylation status of the nucleotide cystine across the entire genome.
WGBS combines sodium bisulfite conversion of the sequence with high throughput DNA sequencing. The sodium bisulfite reaction protects methylcytosines from conversion, whereas unmethylated cytosines are converted into uracil.
Why Is Whole-Genome Bisulfite Sequencing Important?
The ability to characterize an entire organism’s methylation status offers possibilities in all fields of science. WGBS aids research in epigenome-wide studies by showing methylation status genome-wide. It can be used to identify gene expression regulation and transcription modification, which can identify different cell types and disease states.
What Can You Do?
Look Beyond the Genome
Go one step further from the gene sequence and study the transcriptional repression via methylation status
Analyze CpG Sites
Discover gene silencing mechanisms by identifying CpG islands in promoter regions, and profile single nucleotide changes of CpG, CHG, and CHH
Genome-Wide Studies
Study epigenetic mechanisms that regulate processes across the entire genome
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Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes From Our Clients
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
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Beyond WGBS
WGBS isn't the only option for epigenomics studies! Take a look at other options to find the right perspective for your research.
