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Omics Research > Transcriptomics 

Single-Cell Sequencing

Advance gene expression research in suspensions, biological fluids, tissues, or cell populations with Psomagen’s single-cell sequencing technologies.

Let's Talk About SCS

 

What Is Single-Cell Sequencing?

 

Single-cell sequencing interrogates individual cells, specifically the transcriptomes within a heterogeneous cellular environment.

 

Single-cell RNA sequencing allows researchers to examine the small complexities of each region of the transcriptome.

 

Why Is Single Cell Sequencing Important?


With optimized NGS single-cell sequencing (SCS) technologies, researchers can investigate cellular functions to better understand cell-to-cell differentiation, cell lineage relationships, and disease evolution.


Single-cell sequencing can also help in discovery of potential biomarkers and drug targets as well as analysis of rare cell types. The technology
 interrogates individual cells, specifically the transcriptomes within a heterogeneous cellular environment.

 

Single-cell RNA sequencing allows researchers to examine the small complexities of each region of the transcriptome.

What Can You Do?

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3’ and 5’ Gene Expression

Depending on the experimental targets, choose from several options for analyzing a single cell’s RNA.

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Characterize Cell Differences

Find tissue and tumor heterogeneity that can impact the effectiveness of drugs and the rarity of transcripts.

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Discovery for Precision Medicine

Uncover new biomarkers using single-cell transcriptomics. Monitor cell populations during drug development and disease progression.

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Obtain Multi-Dimensional Readouts

Access Next GEM-based technology, which uses advanced microfluidics to perform multiplexed molecular barcoding and single-cell partitioning.

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We're Certified!

 

Did you know we're a 10x Genomics Certified Service Provider (CSP)?

 

We’ve been fully trained and are committed to yearly recertification for the following 10x Genomics services:

 


Learn About Our Certifications

Single-Cell Sequencing Workflows from 10x Genomics®

  • 10x Genomics® Single Cell 3' or 5' Gene Expression

  • 10x Genomics® Single Cell Immune Profiling with or without Gene Expression

  • 10x Genomics® Single Cell ATAC Sequencing

  • 10x Genomics® Single Cell Multiome ATAC with 3’ Gene Expression

Flex with Psomagen + 10x Genomics

The 10x Flex GEX assay makes 10x Genomics single cell sequencing easy and convenient! The Flex GEX is optimized for targeting RNA from fixed tissues (PFA and FFPE), cells, nuclei, and FACS-sorted cells. 

You can: 

  • Fix cells or tissue at the point of collection and batch for later
  • Run assays on your schedule
  • Simplify safe transport
  • Optimize your workflow

Single-Cell Sequencing Best Practices

Sequencing Depth

Psomagen standardly runs single cell sequencing depth based upon the number of cells and the number of sequencing reads per cell. Please inquire for your specific experimental design.

Instrumentation & Read Length

Illumina NovaSeq 6000

  • SP flow cell (650M single reads) 2 x 100 bp
  • S1 flow cell (1,300M single reads) 2 x 100 bp
  • S2 flow cell (3,300M single reads) 2 x 100 bp
  • S4 lane (2,000M single reads) 2 x 150 bp 
  • mkfastq will be provided per 10X recommended read length

 

Illumina HiSeq X

  • V2.5 lane (375 M single reads) 2 x 150 bp 
  • mkfastq will be provided per 10X recommended read length

 

Data Delivery

All sequencing run data is delivered as demultiplexed .fastq files

 

When an entire flow cell or lane is purchased, raw data can be delivered as BCL files if requested at the time of purchase

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!

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1. Request a Consultation
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2. Place Your Order
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3. QC and Data Delivery
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On-Going Promotions

 

We're always adding new deals — check in often for updated promotions!

 

Learn More →

Why Our Clients Choose Psomagen

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Superior Data Quality

Exceeds manufacturer benchmarks

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Fast Turnaround

Starting at one week for sequencing projects

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US-Based Processing

Sequencing and customer support in Rockville, MD

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Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system

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Automated Workflows

Increase scalability and improve reproducibility

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CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)

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Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

Quotes from Our Clients

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PSO Certified!

No one else is a 10x CSP for in all five SCS services.

Start a Project

PSO Much More RNA Sequencing

Choose from a wide portfolio of 10x Genomics platform services, plus many different types of sequencing for various RNA species.

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Total RNA Sequencing
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Bulk mRNA Sequencing
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Small RNA sequencing
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