Omics Research > Transcriptomics

Single-Cell Sequencing

Advance gene expression research in suspensions, biological fluids, tissues, or cell populations with Psomagen’s single-cell sequencing technologies.

What Is Single-Cell Sequencing?

Single-cell sequencing interrogates individual cells, specifically the transcriptomes within a heterogeneous cellular environment.

Single-cell RNA sequencing allows researchers to examine the small complexities of each region of the transcriptome.

Why Is Single Cell Sequencing Important?

With optimized NGS single-cell sequencing (SCS) technologies, researchers can investigate cellular functions to better understand cell-to-cell differentiation, cell lineage relationships, and disease evolution.

Single-cell sequencing can also help in discovery of potential biomarkers and drug targets as well as analysis of rare cell types. The technology interrogates individual cells, specifically the transcriptomes within a heterogeneous cellular environment.

Single-cell RNA sequencing allows researchers to examine the small complexities of each region of the transcriptome.

What Can You Do?

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3’ and 5’ Gene Expression

Depending on the experimental targets, choose from several options for analyzing a single cell’s RNA.

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Characterize Cell Differences

Find tissue and tumor heterogeneity that can impact the effectiveness of drugs and the rarity of transcripts.

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Discovery for Precision Medicine

Uncover new biomarkers using single-cell transcriptomics. Monitor cell populations during drug development and disease progression.

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Obtain Multi-Dimensional Readouts

Access Next GEM-based technology, which uses advanced microfluidics to perform multiplexed molecular barcoding and single-cell partitioning.

Single-Cell Sequencing Workflows from 10x Genomics®

10x Genomics® Single Cell 3' or 5' Gene Expression

Best for:

Profiling the entire transcriptome of individual cells from a heterogeneous population

Benefits:

Single-cell RNA expression levels provide insight into a cell subpopulation’s contribution to overall expression as well as the impact of cellular level mutation and rare transcript abundance
10x Genomics® Single Cell Immune Profiling with or without Gene Expression

Best for:

Studying the variability, differences, and recombination in individual B cells and T cells to investigate the diversity of antibodies in the adaptive immune system

Benefits:

Understanding these rearrangements along with 5’ gene expression from the same cell provides a multi-omic approach to understanding the complex world of immunology and discovering new targets for disease treatment
10x Genomics® Single Cell ATAC Sequencing

Best for:

Assessing the availability of chromatin for interaction with proteins, namely transcription factors, at a single-cell level using the assay for transposase accessible chromatin

Benefits:

Determine the prevalence of subpopulations and their overall impact on a heterogeneous cell sample – a critical area of regulatory pathway discovery
10x Genomics® Single Cell Multiome ATAC with 3’ Gene Expression

Best for:

scATAC sequencing coupled with single-cell 3’ gene expression from the same cell, for solving research and discovery problems at a systems biology level

Benefits:

A multiomics approach to decipher what gene expression is occurring and determine how the structure of that DNA may regulate that expression.

Psomagen was the first 10x Certified Service Provider for scATACSeq in the US and the second worldwide.

Flex with Psomagen + 10x Genomics

The 10x Flex GEX assay makes 10x Genomics single cell sequencing easy and convenient! The Flex GEX is optimized for targeting RNA from fixed tissues (PFA and FFPE), cells, nuclei, and FACS-sorted cells.

You can:

Fix cells or tissue at the point of collection and batch for later
Run assays on your schedule
Simplify safe transport
Optimize your workflow

Single-Cell Sequencing Best Practices

Sequencing Depth

Psomagen standardly runs single cell sequencing depth based upon the number of cells and the number of sequencing reads per cell. Please inquire for your specific experimental design.

Instrumentation & Read Length

Illumina NovaSeq 6000

SP flow cell (650M single reads) 2 x 100 bp
S1 flow cell (1,300M single reads) 2 x 100 bp
S2 flow cell (3,300M single reads) 2 x 100 bp
S4 lane (2,000M single reads) 2 x 150 bp
mkfastq will be provided per 10X recommended read length

Illumina HiSeq X

V2.5 lane (375 M single reads) 2 x 150 bp
mkfastq will be provided per 10X recommended read length

Data Delivery

All sequencing run data is delivered as demultiplexed .fastq files

When an entire flow cell or lane is purchased, raw data can be delivered as BCL files if requested at the time of purchase

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!

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1. Request a Consultation

Create an account through our client portal and get in touch with your local field team to start designing a project.

We’ll help drive your research forward with the right tools for your research needs.

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2. Place Your Order

After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.

Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.

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3. QC and Data Delivery

All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.

You can also see some project information and securely view all valid reports on the client portal site.

Why Our Clients Choose Psomagen

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Superior Data Quality

Exceeds manufacturer benchmarks

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Fast Turnaround

Starting at one week for sequencing projects

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US-Based Processing

Sequencing and customer support in Rockville, MD

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Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system

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Automated Workflows

Increase scalability and improve reproducibility

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CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)

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Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

Quotes from Our Clients

"Excellent service and value for money. Great customer support when issues arise."

Byron Lee, MD, PhD

Cleveland Clinic

"Psomagen provided quick, high-quality service, good after care sales, and the service is valuable for the cost. I recommend them!"

Jinhu Yin, PhD

NCI/NIH

"Competitive pricing, flexibility and high quality. Very good service. Fast and on-time delivery of data."

Ahmad Osman, PhD

Fluent Biosciences, Inc.

PSO Certified!

No one else is a 10x CSP for in all five SCS services.

PSO Much More RNA Sequencing

Choose from a wide portfolio of 10x Genomics platform services, plus many different types of sequencing for various RNA species.

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Total RNA Sequencing

For the most comprehensive data on the whole transcriptome. Encompass all RNA types and sizes recoverable via total RNA sequencing.

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Bulk mRNA Sequencing

A great way to focus on key areas.Sequence all mRNA encoding for protein and having incurred post-transcriptional modification to cover 85% of genetic variation.

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Small RNA sequencing

A more efficient way to analyze RNA. All RNA under 200 bp is typically sequenced with our processes. Depending on platform choice you can analyze multiple variations of coding, non-coding, and regulatory small RNAs.