

Omics Research > Transcriptomics
Single-Cell Sequencing
Advance gene expression research in suspensions, biological fluids, tissues, or cell populations with Psomagen’s single-cell sequencing technologies.
What Is Single-Cell Sequencing?
Single-cell sequencing interrogates individual cells, specifically the transcriptomes within a heterogeneous cellular environment.
Single-cell RNA sequencing allows researchers to examine the small complexities of each region of the transcriptome.
Why Is Single Cell Sequencing Important?
With optimized NGS single-cell sequencing (SCS) technologies, researchers can investigate cellular functions to better understand cell-to-cell differentiation, cell lineage relationships, and disease evolution.
Single-cell sequencing can also help in discovery of potential biomarkers and drug targets as well as analysis of rare cell types. The technology interrogates individual cells, specifically the transcriptomes within a heterogeneous cellular environment.
Single-cell RNA sequencing allows researchers to examine the small complexities of each region of the transcriptome.
What Can You Do?
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3’ and 5’ Gene Expression
Depending on the experimental targets, choose from several options for analyzing a single cell’s RNA.
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Characterize Cell Differences
Find tissue and tumor heterogeneity that can impact the effectiveness of drugs and the rarity of transcripts.
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Discovery for Precision Medicine
Uncover new biomarkers using single-cell transcriptomics. Monitor cell populations during drug development and disease progression.
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Obtain Multi-Dimensional Readouts
Access Next GEM-based technology, which uses advanced microfluidics to perform multiplexed molecular barcoding and single-cell partitioning.

We're Certified!
Did you know we're a 10x Genomics Certified Service Provider (CSP)?
We’ve been fully trained and are committed to yearly recertification for the following 10x Genomics services:
- 3’ Gene Expression
- 5’ Gene Expression
- Flex Fixed Gene Expression
- Immune Profiling
- Multiome ATAC + Gene Expression*
- ATAC
Single-Cell Sequencing Workflows from 10x Genomics®
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10x Genomics® Single Cell 3' or 5' Gene Expression
Best for:
Profiling the entire transcriptome of individual cells from a heterogeneous population
Benefits:
Single-cell RNA expression levels provide insight into a cell subpopulation’s contribution to overall expression as well as the impact of cellular level mutation and rare transcript abundance
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10x Genomics® Single Cell Immune Profiling with or without Gene Expression
Best for:
Studying the variability, differences, and recombination in individual B cells and T cells to investigate the diversity of antibodies in the adaptive immune system
Benefits:
Understanding these rearrangements along with 5’ gene expression from the same cell provides a multi-omic approach to understanding the complex world of immunology and discovering new targets for disease treatment
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10x Genomics® Single Cell ATAC Sequencing
Best for:
Assessing the availability of chromatin for interaction with proteins, namely transcription factors, at a single-cell level using the assay for transposase accessible chromatin
Benefits:
Determine the prevalence of subpopulations and their overall impact on a heterogeneous cell sample – a critical area of regulatory pathway discovery
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10x Genomics® Single Cell Multiome ATAC with 3’ Gene Expression
Best for:
scATAC sequencing coupled with single-cell 3’ gene expression from the same cell, for solving research and discovery problems at a systems biology level
Benefits:A multiomics approach to decipher what gene expression is occurring and determine how the structure of that DNA may regulate that expression.
Psomagen was the first 10x Certified Service Provider for scATACSeq in the US and the second worldwide.
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Flex with Psomagen + 10x Genomics
The 10x Flex GEX assay makes 10x Genomics single cell sequencing easy and convenient! The Flex GEX is optimized for targeting RNA from fixed tissues (PFA and FFPE), cells, nuclei, and FACS-sorted cells.
You can:
- Fix cells or tissue at the point of collection and batch for later
- Run assays on your schedule
- Simplify safe transport
- Optimize your workflow
Single-Cell Sequencing Best Practices
Sequencing Depth
Psomagen standardly runs single cell sequencing depth based upon the number of cells and the number of sequencing reads per cell. Please inquire for your specific experimental design.
Instrumentation & Read Length
Illumina NovaSeq 6000
- SP flow cell (650M single reads) 2 x 100 bp
- S1 flow cell (1,300M single reads) 2 x 100 bp
- S2 flow cell (3,300M single reads) 2 x 100 bp
- S4 lane (2,000M single reads) 2 x 150 bp
- mkfastq will be provided per 10X recommended read length
Illumina HiSeq X
- V2.5 lane (375 M single reads) 2 x 150 bp
- mkfastq will be provided per 10X recommended read length
Data Delivery
All sequencing run data is delivered as demultiplexed .fastq files
When an entire flow cell or lane is purchased, raw data can be delivered as BCL files if requested at the time of purchase
We're Ready for Your Order!
Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!
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1. Request a Consultation
Create an account through our client portal and get in touch with your local field team to start designing a project.
We’ll help drive your research forward with the right tools for your research needs.
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2. Place Your Order
After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.
Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.
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3. QC and Data Delivery
All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.
You can also see some project information and securely view all valid reports on the client portal site.

On-Going Promotions
We're always adding new deals — check in often for updated promotions!
Why Our Clients Choose Psomagen
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Superior Data Quality
Exceeds manufacturer benchmarks
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Fast Turnaround
Starting at one week for sequencing projects
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US-Based Processing
Sequencing and customer support in Rockville, MD
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Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
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Automated Workflows
Increase scalability and improve reproducibility
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CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
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Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes from Our Clients

"Excellent service and value for money. Great customer support when issues arise."
Byron Lee, MD, PhD
Cleveland Clinic

"Psomagen provided quick, high-quality service, good after care sales, and the service is valuable for the cost. I recommend them!"
Jinhu Yin, PhD
NCI/NIH

"Competitive pricing, flexibility and high quality. Very good service. Fast and on-time delivery of data."
Ahmad Osman, PhD
Fluent Biosciences, Inc.
Single-Cell in Action
PSO Much More RNA Sequencing
Choose from a wide portfolio of 10x Genomics platform services, plus many different types of sequencing for various RNA species.