Long-Read Sequencing

What Is Long-Read Sequencing Technology?

Long-read sequencing involves the sequencing of DNA or RNA molecules with extended read lengths, typically exceeding tens of thousands base pairs.

PacBio's long-read technology uses a single DNA molecule, thousands of nucleotides long, to produce individual DNA or RNA reads up to 20,000 bases. This can include information about epigenetic modifications — without extra bioinformatics steps.

Why Is Long-Read Sequencing Important?

Extended read length allows researchers to capture complex genomic regions, characterize structural variations, identify epigenetic modifications, and decode hard-to-sequence regions.

This can generate more accurate genome assemblies, better understanding of genetic variations, and enhanced insights into various biological processes. These benefits have applications from medicine to genomics research.

What Can You Do?

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Comprehensive Genome Assembly

Reconstruct or generate de novo genomes with fewer errors and areas of uncertainty, even in areas of high homology.

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Haplotype Phasing

Obtain a complete and accurate representation of each copy of the genome or region of interest.

Variant Detection

Reach difficult to detect genomic regions, like high GC regions or large insertion-deletion areas. Link phenotypes to novel genes and causative variants.

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Epigenetics

Get methylation data from your sample without extra steps or protocols. Take simultaneous advantage of haplotype-phased and variant-called genomic information.

Our Services

Whole Genome Sequencing

Obtain the complete DNA information of an organism, encompassing even the most challenging-to-sequence regions, with remarkable precision and unbiased representation. Identify genetic variations, perform de novo sequencing, analyze structural genomic changes, and investigate epigenetic details.

More about WGS →
Single-Cell Transcriptomics

Uncover the diversity of RNA at the isoform level, which holds immense importance for both fundamental research and studying diseases.

With PacBio MAS-Seq, you can sequence entire RNA isoforms within individual cells, accompanied by unique single-cell barcodes and UMI data. This approach provides unparalleled insights into the intricacies of single-cell biology.

More on Single-Cell →
Targeted Sequencing

Using Twist Bioscience's target enrichment kits in conjunction with PacBio's long-reads technology, you gain an adaptable and cost-effective solution for achieving comprehensive coverage of challenging genomic regions.

This method offers a robust alternative for scaling up your sequencing efforts.
Metagenomics

Access details about microbial communities and microbiomes with shotgun metagenomic profiling and assembly using long-read sequencing. Accurately characterize the functional aspects and taxonomy of microbial communities or access full-length 16S sequences to acquire information at the species/strain level.

More on Metagenomics →

Run Your Long Reads on the Revio

Psomagen has PacBio's latest sequencing instrument — see what you can discover with a 15x increase in read throughput. Plus, get exclusive deals through 2023 when you make a long-read sequencing order, or combine long-reads with other NGS technologies.

About Revio →

Long-Read Best Practices

Sample Types

We accept numerous sample types for our long-read technology, not limited to:

Cultured cells and bacteria
Blood
Plants
Tissue

Regardless of the starting material, it is possible to attain high quality, accurate results. We also offer extraction services for your convenience.

Expected Output

Each SMRT Cell can produce up to 90 Gb of total long-read sequencing data, a phased 30x whole human genome, up to 80 million full-length RNA isoforms, and approximately 170 HQ-MAGs from a human gut microbiome sample.

Data Delivery

Standard report includes sequence data and QC results. Depending on your needs, we can include variant calling, mapped files, methylation information and more.

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have questions during sequencing, it's easy to get in touch!

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1. Request a Consultation

Get in touch with your local field team for a project quote. We'll help drive your research forward with the right tools for your research needs.

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2. Place an Order

Log in to your account and make an order through our online portal. Your payment method and sample delivery method should be determined during the order registration.

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3. Submit Your Samples

Submit your samples via your selected delivery method.

Interested in same-day services? Be sure to confirm the cut-off time for sample submission at your location.

Why Our Customers Choose Psomagen

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Superior Data Quality

Exceeds manufacturer benchmarks

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Fast Turnaround

16 to 24 hours for WPS

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US-Based Processing

Sequencing and customer support in Rockville, MD

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Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system

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Automated Workflows

Increase scalability and improve reproducibility

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CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)

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Complete Sequencing Solutions

From extraction to data analysis, with many data types accepted

PSO Multiomic!

How multiomic is your approach? See how our service offerings can work together for research like never before.

Third Generation Sequencing Is Here!

See how the newest technologies are providing deeper insights in omics research.

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Spatial Biology

See what you can discover with the ultimate systems biology tool. Uncover mechanisms at the level of organs, tissues, cells, and even sub-cellular structures.

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Single-Cell Sequencing

Advance your gene expression research in suspensions, biological fluids, tissues, or cell populations.