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What Is Long-Read Sequencing Technology?
Long-read sequencing involves the sequencing of DNA or RNA molecules with extended read lengths, typically exceeding tens of thousands base pairs.
PacBio's long-read technology uses a single DNA molecule, thousands of nucleotides long, to produce individual DNA or RNA reads up to 20,000 bases. This can include information about epigenetic modifications — without extra bioinformatics steps.
Why Is Long-Read Sequencing Important?
Extended read length allows researchers to capture complex genomic regions, characterize structural variations, identify epigenetic modifications, and decode hard-to-sequence regions.
This can generate more accurate genome assemblies, better understanding of genetic variations, and enhanced insights into various biological processes. These benefits have applications from medicine to genomics research.
What Can You Do?
Comprehensive Genome Assembly
Reconstruct or generate de novo genomes with fewer errors and areas of uncertainty, even in areas of high homology.
Obtain a complete and accurate representation of each copy of the genome or region of interest.
Reach difficult to detect genomic regions, like high GC regions or large insertion-deletion areas. Link phenotypes to novel genes and causative variants.
Get methylation data from your sample without extra steps or protocols. Take simultaneous advantage of haplotype-phased and variant-called genomic information.
Whole Genome Sequencing
Obtain the complete DNA information of an organism, encompassing even the most challenging-to-sequence regions, with remarkable precision and unbiased representation. Identify genetic variations, perform de novo sequencing, analyze structural genomic changes, and investigate epigenetic details.
Uncover the diversity of RNA at the isoform level, which holds immense importance for both fundamental research and studying diseases.
With PacBio MAS-Seq, you can sequence entire RNA isoforms within individual cells, accompanied by unique single-cell barcodes and UMI data. This approach provides unparalleled insights into the intricacies of single-cell biology.
Using Twist Bioscience's target enrichment kits in conjunction with PacBio's long-reads technology, you gain an adaptable and cost-effective solution for achieving comprehensive coverage of challenging genomic regions.
This method offers a robust alternative for scaling up your sequencing efforts.
Access details about microbial communities and microbiomes with shotgun metagenomic profiling and assembly using long-read sequencing. Accurately characterize the functional aspects and taxonomy of microbial communities or access full-length 16S sequences to acquire information at the species/strain level.
Long-Read Best Practices
We accept numerous sample types for our long-read technology, not limited to:
- Cultured cells and bacteria
Regardless of the starting material, it is possible to attain high quality, accurate results. We also offer extraction services for your convenience.
Each SMRT Cell can produce up to 90 Gb of total long-read sequencing data, a phased 30x whole human genome, up to 80 million full-length RNA isoforms, and approximately 170 HQ-MAGs from a human gut microbiome sample.
Standard report includes sequence data and QC results. Depending on your needs, we can include variant calling, mapped files, methylation information and more.
We're Ready for Your Order!
Here's what to expect in your project's life cycle. If you have questions during sequencing, it's easy to get in touch!
1. Request a Consultation
Get in touch with your local field team for a project quote. We'll help drive your research forward with the right tools for your research needs.
2. Place an Order
3. Submit Your Samples
Submit your samples via your selected delivery method.
Interested in same-day services? Be sure to confirm the cut-off time for sample submission at your location.
Why Our Customers Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
16 to 24 hours for WPS
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many data types accepted
Third Generation Sequencing Is Here!
See how the newest technologies are providing deeper insights in omics research.