What Is Long-Read Sequencing Technology?
Long-read sequencing involves the sequencing of DNA or RNA molecules with extended read lengths, typically exceeding tens of thousands base pairs.
PacBio's long-read technology uses a single DNA molecule, thousands of nucleotides long, to produce individual DNA or RNA reads up to 20,000 bases. This can include information about epigenetic modifications — without extra bioinformatics steps.
Why Is Long-Read Sequencing Important?
Extended read length allows researchers to capture complex genomic regions, characterize structural variations, identify epigenetic modifications, and decode hard-to-sequence regions.
This can generate more accurate genome assemblies, better understanding of genetic variations, and enhanced insights into various biological processes. These benefits have applications from medicine to genomics research.
What Can You Do?
Comprehensive Genome Assembly
Reconstruct or generate de novo genomes with fewer errors and areas of uncertainty, even in areas of high homology.
Haplotype Phasing
Obtain a complete and accurate representation of each copy of the genome or region of interest.
Variant Detection
Reach difficult to detect genomic regions, like high GC regions or large insertion-deletion areas. Link phenotypes to novel genes and causative variants.
Epigenetics
Get methylation data from your sample without extra steps or protocols. Take simultaneous advantage of haplotype-phased and variant-called genomic information.
Our Services
Whole Genome Sequencing 
Obtain the complete DNA information of an organism, encompassing even the most challenging-to-sequence regions, with remarkable precision and unbiased representation. Identify genetic variations, perform de novo sequencing, analyze structural genomic changes, and investigate epigenetic details.
Single-Cell Transcriptomics
Uncover the diversity of RNA at the isoform level, which holds immense importance for both fundamental research and studying diseases.
With PacBio MAS-Seq, you can sequence entire RNA isoforms within individual cells, accompanied by unique single-cell barcodes and UMI data. This approach provides unparalleled insights into the intricacies of single-cell biology.
Targeted Sequencing
Using Twist Bioscience's target enrichment kits in conjunction with PacBio's long-reads technology, you gain an adaptable and cost-effective solution for achieving comprehensive coverage of challenging genomic regions.
This method offers a robust alternative for scaling up your sequencing efforts.
Metagenomics
Access details about microbial communities and microbiomes with shotgun metagenomic profiling and assembly using long-read sequencing. Accurately characterize the functional aspects and taxonomy of microbial communities or access full-length 16S sequences to acquire information at the species/strain level.
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Third Generation Sequencing Is Here!
See how the newest technologies are providing deeper insights in omics research.
