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What is Sanger Sequencing?
Sanger Sequencing is the oldest proven process of sequencing regions of DNA by using oligonucleotide primers and chain termination.
It is commonly selected to sequence polymerase chain reaction (PCR) products, plasmid DNA, and bacterial artificial chromosomes (BAC).
Why is Studying Sanger Sequencing Important?
The original sequencing method preceding NGS, Sanger sequencing is still useful as a low-cost, high accuracy method for interrogating small DNA regions in a limited number of samples.
What Can You Do?
Verify correct insertion and orientation into plasmid and bacterial artificial chromosome (BAC) backbones.
Use Capillary Electrophoresis
Get quicker, more accurate services via automated capillary electrophoresis sequencing (CES).
Access Sequencing Expertise
Enlist Psomagen’s expert help as needed before, during, and after service.
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Learn how Psomagen’s barcode-based ordering system reduces form fills.
Tools for Sanger Sequencing
Standard Sanger Sequencing
Custom Sanger Sequencing
Quotes From Our Clients
"Their service is always on time. They are super patient with orders."
"It's my great pleasure to use them. I've been a customer of Psomagen for a long time and for all of my supplies. I cannot express in words how satisfied I am. My orders are always on time and the staff services are so friendly and efficient. They are very helpful when I have questions and are generous with giving any kind of information. I appreciate Psomagen sequencing and all they do for me. I hope we will be together for our whole tenure. They definitely did a wonderful job!"
Amit Kumar Singh, Ph.D.
"I have used Psomagen for sequencing my clones for a site-directed mutagenesis screen. I enjoy the speed of delivery and the fact that I get my results before I wake up the next day. Customer care is present to answer all questions and is quick to resolve issues. I believe this is value for the money."
Rutgers University, New-Brunswick
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Get in touch with your local field team and start designing a project. We’ll help drive your research forward with the right tools for your investigative needs.
Create, Manage and Track Your Project
At this step you'll be able to keep an eye on all the various methods and platforms we’re using to ensure your starting material will produce the most in-depth information possible. It’s easy to get in touch with us if you have a question or concern.
Get Your Data and Analysis Reports
Securely access your raw data and view detailed analyses specific to your scientific question all in one place. Then reach out to our bioinformatics scientists to dig deeper into the results.
Generation of iPSC line from a Parkinson patient with PARK7 mutation and CRISPR-edited Gibco human episomal iPSC line to mimic PARK7 mutation
Step One Sanger, Step Two NGS
While Sanger sequencing is tried and true, some projects require the massively parallel sequencing provided by our NGS offerings.