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Omics Research // Genomics

Bacterial Genome Sequencing

Understand pathogen genetics and identify targets for treatment with bacterial genome sequencing using long-read sequencing technology.

What Is Bacterial Genome Sequencing?

Bacterial genome sequencing (BGS) is a sequencing method used to characterize bacterial pathogens. This methodology provides insights into virulence, antibiotic resistance, and other properties important to researchers. 


Why Is Bacterial Genome Sequencing Important?

Bacterial diseases are a major cause of illness and morbidity in humans and animals. Increased incidence of antibiotic resistance makes it important to identify mutations and develop additional treatments.

Long reads are a valuable tool for bacterial genome sequencing. R10.4.1 flow cells can generate near-complete bacterial genomes. This eliminates the need for short-read assembly, which can struggle to resolve highly repetitive regions. 

What Can You Do?

Classify Bacteria

Find evolutionary links between strains of bacteria. Classify unknown microbes. 


Monitor Outbreaks

Identify bacteria and mutations responsible for infectious disease outbreaks. 


Explore Microbial Diversity

Understand bacterial diversity in an environment.


Personalized Medicine

Characterize host-pathogen interactions and develop appropriate treatments.

Bacterial Genome Sequencing Best Practices

Sample Requirements

  • Sample type: single species bacterial DNA
  • Concentration: 100–150 ng/µl
  • Purity: A260/280 = 1.8–2.0
  • Volume: 15 µl/sample
  • Size: Up to 12M bases

Sample Size

Small (1~6Mb)

  • Concentration: 100–150 ng/µl
  • Volume: 15 µl
  • Coverage: 100x
  • Price: $90/sample

Large (6~12Mb)

  • Concentration: 100-150 ng/µl
  • Volume: 15 µl
  • Coverage: 100x
  • Price: $110/sample


v14 library prep chemistry paired with R10.4.1 flow cells, raw data accuracy is >99%

Quality Check

Targeting data size based on the targeting coverage. Any results that do not meet the targeting data size can be translated to insufficient gDNA.


Sequencing results are directly dependent on the quantity, quality, and purity of sample DNA.


File types: 

  • FASTA (consensus sequence of the genome)
  • GenBank (.gbk file)
  • FASTQ (all the raw sequencing reads)

Turnaround Time

Small sample (<6Mb): 1–2 business days

Large sample (<12Mb): 3–4 business days

gDNA extraction service: additional 2–3 business days (coming soon)


Causes of inadequate sample quality: 

  • Insufficient DNA concentration
    • Verification on Qubit
  • Degraded and/or contaminated samples
    • Poor gDNA extraction technique
    • Reagents remaining in final product
    • Multiple freeze/thaw cycles
    • Exposure to extreme temperatures
  • Multi-species sample
    • This service is intended for single-species bacterial samples. Non-bacterial or multi-species samples may fail. 

Why Our Clients Choose Psomagen


Superior Data Quality

Exceeds manufacturer benchmarks


Fast Turnaround

Starting at one week for sequencing projects


US-Based Processing

Sequencing and customer support in Rockville, MD


Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system


Automated Workflows

Increase scalability and improve reproducibility


CLIA-Certified, CAP-Accredited Labs

Exceeds manufacturer benchmarks


Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted