How It Works
The 10x Genomics Kinnex single-cell RNA kit turns amplicons into larger fragment libraries. This MAS-Seq based sample prep technology makes libraries compatible with PacBio long-read instruments. Now, it's possible to generate HiFi single-cell data!
The MAS-Seq Advantage
Short read-sequencing library preparation protocols include fragmentation steps which shear full length cDNA, yielding smaller molecules which are more suitable for short read sequencing. These short fragments require transcript assembly to combine reads into a full length molecule.
As a result, RNA processing steps, like alternative splicing or fusion events, cannot be accurately detected using short read sequencing.
Long read sequencing facilitates sequencing of the entire length of the cDNA molecule, including all exonic regions and fusion sites. Kinnex library preparation methods use a simple PCR reaction to add small adapters to the end of full length cDNA molecules. The adapter on one transcript anneals to the adapter on another, yielding a chain of cDNA molecules connected by short, known sequences. A final SMRTbell library can contain up to 12 full-length transcripts.
PacBio Benefits
Full-length isoform information
16x throughput increase vs. other single-cell long read kits
Cellular heterogeneity meets unmatched accuracy
About the Kinnex Single-Cell RNA Kit
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Number of reactions |
12 |
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Target throughput |
Revio: 100-120 million reads |
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Multiplexing |
4-plex |
More Single-Cell at Psomagen
Our labs are always adding more technologies and capabilities to our Spatial Biology & Single-Cell Center of Excellence. This means you'll be able to find the platform that suits your project. Take a look at other options below.
