What Is Reduced Representation Bisulfite Sequencing (RRBS)?
RRBS is used to get a “reduced representation" of the genome, with a focus on CpG islands. The restriction enzyme is used to digest the DNA during the fragmentation step. It enables us to profile genome-wide methylation on a single nucleotide scale using a MspI restriction enzyme digestion at CpG sites.
RRBS is an alternative to whole genome bisulfite sequencing (WGBS) that requires less sequencing coverage from a single sample.
Why Is Whole-Genome Bisulfite Sequencing Important?
RRBS enriches regions of high methylation, resulting in a focus on the regions of most interest. RRBS aids research in epigenomic-wide studies by studying the methylation status at CpG locations. It can be used to identify gene expression regulation and transcription modification that is used to identify different cell types and disease states.
Using RRBS, the methylation status of cysteine residues can be identified. The genomic region of the CpG and its function can also be identified and reported.
What Can You Do?
Focus Your Methylation Studies
Study areas of high methylation at CpG dinucleotides by using a restriction enzyme digestion
Reduce Costs, Lower Sequencing Coverage
Use less raw sequencing throughput than WGBS to study the methylation that plays an important role in regulation of transcription
Genome-Wide Studies Across Multiple Applications
Study regions of high methylation across the entire genome to inform on epigenomic changes in fields of immunology, cancer, and neuroscience
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Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes From Our Clients
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
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Go Beyond RRBS
There's other options for your epigenomics research! Find the right fit for you with other Psomagen services.
