Whole Plasmid Sequencing

What Is Whole Plasmid Sequencing?

With the help of cutting-edge, long-read sequencing technologies, whole plasmid seq allows for the complete sequencing of a plasmid with accuracy and efficiency. This technique does not require primers.

Why Is Whole Plasmid Sequencing Important?

With whole plasmid sequencing, research goes beyond the scope of what's possible for Sanger sequencing. Rather than examining a small, specific area of the plasmid, whole plasmid sequencing gets the full picture. This opens the door to additional insights and research opportunities.

What Can You Do?

Maximize Your Budget

Faster and lower cost than standard primer walking service.

Visualize the Entire Plasmid

Attain accurate and reliable confirmation of plasmid sequences.

New & Improved Technology

Use the newest V14 chemistry and updated R10.4.1 Nanopore flow cell to provide the highest accuracy.

Whole Plasmid Sequencing Best Practices

Sample Requirements

Sample Type: Plasmid DNA
Length: Up to 300 Kb
Concentration: 50-100 ng/µl
Volume: Minimum 15 µl per sample
Purity (A260/280): 1.8 – 2.0

Sample Size

Small (1~30 kb)

Concentration: 50-100 ng/µl
Volume: 15 µl
Turnaround time: 16 to 24 hours
Price: $15/sample

Medium (31~150 kb)

Concentration: 50-100 ng/µl
Volume: 15 µl
Turnaround time: 16 to 24 hours
Price: $30/sample

Large (151~300 kb)

Concentration: 50-100 ng/µl
Volume: 15 µl
Turnaround time: 16 to 24 hours
Price: $60/sample

Turnaround Time

Standard WPS: 1 business day
Plasmid isolation service: 2-3 additional business days

Quality Check

Quality score determined by analysis software.
Targeting data size based on the targeting coverage.
Any results that do not meet the targeting data size can be translated into insufficient sample DNA.
Sequencing results are directly dependent on the quantity, quality, and purity of sample DNA.

Coverage

Under 30 kb: 200x
Under 150 kb: 200x
Under 300 kb: 200x

Accuracy

v14 library prep chemistry paired with R10.4.1 flow cells, raw data accuracy is >99%

Assembly and Annotation

This service is intended for plasmid samples. Any non-plasmid sample submission must be sequenced at the client's own risk.

Results

File types:

FASTA (consensus sequence of the genome)
GenBank (.gbk file)
FASTQ (all the raw sequencing reads)

Troubleshooting

Causes of inadequate sample quality:

Insufficient DNA concentration
- Verification on Qubit
Degraded and/or contaminated samples
- Caused by poor plasmid purification technique or reagents remaining in final product.
- Caused by multiple freeze/thaw cycles and/or exposure to extreme temperatures.
Multi-species sample
- This service is intended for clonal plasmid samples. Assembly and analysis of multi-species samples may fail.
- Despite producing final results with multi-species sample, the final assembly and analysis may not be an accurate representation.

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!

01

Request a Consultation

Get in touch with your local field team for a project quote. We'll help drive your research forward with the right tools for your research needs.

02

Place an Order

Log in to your account and make an order through our online portal. Your payment method and sample delivery method should be determined during the order registration.

03

Submit Your Samples

Submit your samples via your selected delivery method.Interested in same-day services? Be sure to confirm the cut-off time for sample submission at your location.

Start an Order Through Our Online Portal

Why Our Clients Choose Psomagen

Superior Data Quality

Exceeds manufacturer benchmarks

Fast Turnaround

Starting at one week for sequencing projects

US-Based Processing

Sequencing and customer support in Rockville, MD

Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system

Automated Workflows

Increase scalability and improve reproducibility

CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)

Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

Get the Whole Omics Story, DNA to Protein

Studying each step in the central dogma reveals valuable insights, but DNA-RNA-Protein together tell the whole biological story. Please also consider these other services for your systems biology investigations.

Sanger Sequencing

With the gold standard of sequencing, get a low-cost, high-accuracy method for your project. Confirm sequences and investigate small DNA regions through a lab with nearly 20 years of experience.

Transcriptomics

A more complete picture of various disease states. Take a closer look at both coding and non-coding RNA — mRNA, smRNA, lncRNA, and more — with a variety of sequencing strategies.

Metagenomics

A new understanding of the world’s biosphere. Discover the biological diversity in environmental and microbial samples and their evolution and function.

Proteomics

A novel way to predict and understand human illness. Go beyond the nucleic acid map and identify and quantify the protein biomarkers that are the hallmarks of disease.

Omics Research // Genomics