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Omics Research // Genomics

Whole Plasmid Sequencing

A new, low-cost sequencing platform that retains
Sanger sequencing's industry-leading turnaround time.

What Is Whole Plasmid Sequencing?

With the help of cutting-edge, long-read sequencing technologies, whole plasmid seq allows for the complete sequencing of a plasmid with accuracy and efficiency. This technique does not require primers.


Why Is Whole Plasmid Sequencing Important?

With whole plasmid sequencing, research goes beyond the scope of what's possible for Sanger sequencing. Rather than examining a small, specific area of the plasmid, whole plasmid sequencing gets the full picture. This opens the door to additional insights and research opportunities.

What Can You Do?

Maximize Your Budget

Faster and lower cost than standard primer walking service.


Visualize the Entire Plasmid

Attain accurate and reliable confirmation of plasmid sequences.


New & Improved Technology

Use the newest V14 chemistry and updated R10.4.1 Nanopore flow cell to provide the highest accuracy.

Whole Plasmid Sequencing Best Practices

Sample Requirements

  • Sample Type: Plasmid DNA
  • Length: Up to 300 Kb
  • Concentration: 50-100 ng/µl
  • Volume: Minimum 15 µl per sample
  • Purity (A260/280): 1.8 – 2.0

Sample Size

Small (1~30 kb)

  • Concentration: 50-100 ng/µl
  • Volume: 15 µl
  • Turnaround time: 16 to 24 hours
  • Price: $15/sample

Medium (31~150 kb)

  • Concentration: 50-100 ng/µl
  • Volume: 15 µl
  • Turnaround time: 16 to 24 hours
  • Price: $30/sample

Large (151~300 kb)

  • Concentration: 50-100 ng/µl
  • Volume: 15 µl
  • Turnaround time: 16 to 24 hours
  • Price: $60/sample

Turnaround Time

  • Standard WPS: 1 business day
  • Plasmid isolation service: 2-3 additional business days

Quality Check

  • Quality score determined by analysis software.
  • Targeting data size based on the targeting coverage.
  • Any results that do not meet the targeting data size can be translated into insufficient sample DNA.
  • Sequencing results are directly dependent on the quantity, quality, and purity of sample DNA.


  • Under 30 kb: 200x
  • Under 150 kb: 200x
  • Under 300 kb: 200x


v14 library prep chemistry paired with R10.4.1 flow cells, raw data accuracy is >99%

Assembly and Annotation

This service is intended for plasmid samples. Any non-plasmid sample submission must be sequenced at the client's own risk. 


File types: 

  • FASTA (consensus sequence of the genome)
  • GenBank (.gbk file)
  • FASTQ (all the raw sequencing reads)


Causes of inadequate sample quality: 

  • Insufficient DNA concentration
    • Verification on Qubit
  • Degraded and/or contaminated samples
    • Caused by poor plasmid purification technique or reagents remaining in final product. 
    • Caused by multiple freeze/thaw cycles and/or exposure to extreme temperatures. 
  • Multi-species sample
    • This service is intended for clonal plasmid samples. Assembly and analysis of multi-species samples may fail.
    • Despite producing final results with multi-species sample, the final assembly and analysis may not be an accurate representation. 

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!


Request a Consultation

Get in touch with your local field team for a project quote. We'll help drive your research forward with the right tools for your research needs.


Place an Order

Log in to your account and make an order through our online portal. Your payment method and sample delivery method should be determined during the order registration.


Submit Your Samples

Submit your samples via your selected delivery method.Interested in same-day services? Be sure to confirm the cut-off time for sample submission at your location.

See How It Works!

Get a free whole plasmid sequencing sample with your first WPS order.

*Offer valid only in the US and Canada. Use one-time promo code WPS0723.


Why Our Clients Choose Psomagen


Superior Data Quality

Exceeds manufacturer benchmarks


Fast Turnaround

Starting at one week for sequencing projects


US-Based Processing

Sequencing and customer support in Rockville, MD


Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system


Automated Workflows

Increase scalability and improve reproducibility


CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)


Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

What's Possible with WPS?

Genetically encoded lysine photocage for spatiotemporal control of TDP-43 nuclear import
Diverse Small Circular DNA Viruses Identified in an American Wigeon Fecal Sample
Development of 2-Aminotetralin-Type Serotinin 5-HT1 Agonists: Molecular Determinants for Selective Binding and Signaling at 5-HT1A, 5-HT1B, 5-HT1D, and 5-HT1F Receptors
PSO Multiomic!

How multiomic is your approach? See how our service offerings can work together for research like never before.