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Omics Research // SINGLE-CELL SEQUENCING

10x Genomics for Single Cell

10x Genomics' single-cell portfolio offers a diverse set of options, making it possible to tailor your next project to your research question. 

Let’s Talk About Single Cell

How It Works

Microfluidics encapsulate an individual cell along with a Gel Bead, coated in uniquely barcoded oligo-dT primers for mRNA capture. This process creates full length cDNA suitable for long read sequencing.

High throughput assays make it possible to capture up to 20,000 cells per sample.

We've Got the Right Option For You!

Whether you're looking to send premixed samples, choose a standard primer, or create your own project, we can help.

3' Gene Expression

Standard gene expression capture. Use gene expression levels to identify cell subtypes and cell trajectories for broad information. 

  • Compatible with full length cDNA sequencing
  • Up to 80% cell recovery
  • Whole transcriptome coverage
Learn More

5' Gene Expression

Gene expression capture, compatible with immune profiling for human and mouse TCR and BCR enriched sequencing and targeted enrichment.

  • Up to 80% cell recovery
  • Whole transcriptome coverage
 
Learn More

Cell Surface Protein Expression

Compatible with 3' and 5' Gene Expression assays.

Use Feature Barcode technology to label surface proteins for a better understanding of their locations, functions, and interactions with other parts of their biological system.

Protocol Details

CRISPR Screening

Compatible with 3' and 5' Gene Expression assays. 

  • Confirm phenotypic effects of CRISPR edits
  • Study developmental mechanisms, disease, gene function, and treatment efficacy
Learn More

Cell Hashing/Multiplexing

Combine multiple pre-labeled samples into a single lane.

Efficiently multiplex samples with this technology, eliminating batch effects and creating signficant cost savings for single-cell projects. 

Learn More

ATAC-Seq

Chromatin accessibility from a single nucleus, assessing its availability for interaction with proteins, namely transcription factors. 

Determine the prevalence of subpopulations and their overall impact on a heterogeneous cell sample – a critical area of regulatory pathway discovery.

Learn More

Multiome

3' gene expression plus ATAC from the same nucleus. A good choice for solving research and discovery problems at a systems biology level. Determine what gene expression is occurring and how the structure of that DNA may regulate that expression.

Learn More

Flex

Probe-based capture from fixed or FFPE human and mouse cells or nuclei. This is an excellent choice for difficult samples, including low input or archival samples. 

Learn More

Choosing an Assay

 

3' Gene Expression

5' Gene Expression

            
 

3' v3.1

3' HT

3' GEM-X

5' v2

5' HT

5' GEM-X

ATAC Seq

Multiome

Flex

      

Targets

RNA Expression (GEx)

GEx and cell surface protein (CSP)

GEx

GEx and CSP

GEx

GEx and CSP

GEx

GEx and CSP

GEx

GEx and CSP

GEx and immune profiling (VDJ)

GEx, CSP, VDJ

GEx

GEx and CSP

GEx and VDJ

GEx, CSP, VDJ

ATAC

ATAC and GEx

GEx

      

Sample multiplexing

Yes

Yes

Yes

Yes

Yes

Yes

Not supported

Not supported

Yes

      

Max samples/run

8

16

8

8

16

8

8

8

8

      

Max cells/sample

10k

20k

20k

10k

20k

20k

10k

10k

10k

      

Max cells/lane

20k

60k

60k

60k

60k

60k

10k

10k

10k

      

Capture rate

60-65%

60-65%

65-70%

60-65%

60-65%

65-70%

60-65%

60-65%

2.00%

      

Input sample type

Cells or nuclei

Cells or nuclei

Nuclei

Nuclei

Fixed* or FFPE cells or nuclei

      

QC Requirement

Viability/DAPI >80%

Viability/DAPI >80%

Viability/DAPI >80%

Viability/DAPI >80%

Viability >80%; DV 200 >50%

      

 

1
4
8
11
13
10

Download a Sample Report

Our new 10x Genomics single-cell sequencing reports provide improved depth of information about your sample results. Now offering cellranger outputs and Seurat analysis including fastq files, RNA expression matrices, cell filtering, normalization, clustering, and cell type identification so you can assess your results before you pull out your statistical tools.

Reach out to inquiry@psomagen.com to start your data analysis, or download a sample PDF below: 

Our Instruments

10x Genomics Chromium X

10x Genomics Chromium X

Chromium X simplifies, automates, and scales single-cell analysis with its proprietary Next Gen technology. Each of these single-cell partitions contains a unique barcode for downstream analysis, allowing researchers to generate millions of single cells. This system ushers in a new standard in flexibility for single cell technology.  The Chromium X brings robust and cost effective scRNAseq and targeted content to Psomagen customers.  

It is now possible to discover previously unattainable biological information when innovative reagent delivery systems are combined with turnkey software analysis tools.

certified

We're Certified!

Did you know we're a 10x Genomics Certified Service Provider (CSP)?

We’ve been fully trained and are committed to yearly recertification for the following 10x Genomics services:

Learn About Our Certifications

More Single-Cell at Psomagen

Our labs are always adding more technologies and capabilities to our Spatial Biology & Single-Cell Center of Excellence. This means you'll be able to find the platform that suits your project. Take a look at other options below.

Fluent Biosciences

Now an Illumina company, Fluent Biosciences offers high-throughput, instrument free emulsion for multiplexing many samples in a single reaction.

BioSkryb Genomics

Allows researchers to capture RNA and DNA from the same cell. Conduct single-cell with high fidelity thanks to Primary Template Amplification technology.

PacBio Long-Read Single Cell

Library preparation for full length, long-read cDNA generated by the 10x Genomics Kinnex single cell kit, which creates a PacBio compatible, SMRTbell library. Enables isoform and fusion detection without the need for assembly.

Scale Biosciences

QuantumScale single-cell RNA offers flexibility and efficiency without sacrificing high sensitivity and quality. Capture and fix up to 4 million cells, ideal for studies with multiple timepoints.

Parse Biosciences

A unique, plate based approach for highly multiplexed cell capture, scalable to your project's needs.