Fluent Biosciences

How PIP-Seq Works

High-throughput, instrument free emulsion capture uses core templated particles coated with a barcoded primer to capture mRNA. Add on Supplemental Enrichment Amplification (SEA) kits to capture cell surface proteins or CRISPR gRNAs and mRNA together.

Scalable kits allow you to pay for exactly the cell capture you need, from hundreds to millions of cells. Fluent single-cell technology is fully compatible with Illumina for all project stages.

Psomagen currently offers sequencing for pre-made PIP-Seq libraries. If you are looking for the complete workflow, please inquire.

Tailor Your Single-Cell Project

Additional Fluent BioSciences Options

SEA

Supplemental Enrichment Amplification (SEA) uses Synthetic Nucleotide Tags (SNTs) to capture protein or CRISPR gRNAs along with 3`mRNA.

Nuclei

Isolation kit designed specifically for Illumina single-cell RNA sample prep, validated on several frozen tissue types. Compatible with all 3' RNA kits.

Supported Methodologies

3' PolyA RNA

SNT (proteins and cell hashing)

Targeted 3'

Cells and nuclei

Fresh and DSP-methanol fixed

Plants and animals

CROPseq

More Single-Cell at Psomagen

Our labs are always adding more technologies and capabilities to our Spatial Biology & Single-Cell Center of Excellence. This means you'll be able to find the platform that suits your project. Take a look at other options below.

10x Genomics

We're a 10x Certified Service Provider for 3' and 5' Gene Expression, ATAC, CRISPR screening, immune profiling, and more.

BioSkryb Genomics

Allows researchers to capture RNA and DNA from the same cell. Conduct single-cell with high fidelity thanks to Primary Template Amplification technology.

PacBio Long-Read Single Cell

Library preparation for full length, long-read cDNA generated by the 10x Genomics Kinnex single cell kit, which creates a PacBio compatible, SMRTbell library. Enables isoform and fusion detection without the need for assembly.

Scale Biosciences

QuantumScale single-cell RNA offers flexibility and efficiency without sacrificing high sensitivity and quality. Capture and fix up to 4 million cells, ideal for studies with multiple timepoints.

Parse Biosciences

A unique, plate based approach for highly multiplexed cell capture, scalable to your project's needs.

	T2	T10	T20	T100
Cell output per reaction	Up to 2,000	Up to 10,000	Up to 20,000	Up to 100,000
Samples per kit	8	8	4	2

Omics Research // SINGLE-CELL SEQUENCING

Fluent BioSciences from Illumina