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Omics Research // Genomics

Targeted Gene Sequencing

Drill down on your human, mouse, rat, and other non-human targets' genetic sequence with amplicon and hybrid capture techniques.

What Is Targeted Sequencing?

Targeted sequencing is a methodology whereby distinct genomic regions of interest to the organism you’re studying are captured or selected for deeper sequencing depth.


Why Is Targeted Sequencing Important?

For scientists only interested in certain regions of the genome, targeted sequencing is more efficient and cost-effective than whole-genome sequencing or whole-exome sequencing and can yield specific, in-depth insights.

It can detect disease-related variants, inherited germline mutations, and CRISPR gene editing events. It can also target well-characterized variants within exons and methylation regions, as well as low-frequency variants.

What Can You Do?

Find Regions of High Interest

Pinpoint the exact region of nucleic acid that can efficiently unlock the answers you seek


Use Multiple Methodologies

A targeted approach and deeper sequencing depth make it easier to identify variants


Discover Disease Variants

Choose the best enrichment strategy — hybrid capture, amplicon, or custom — for your target region


Precision Medicine

Introduce new ways to prevent, diagnose, and treat disease on a personal level

Technologies Used for Targeted Sequencing

Amplicon Sequencing

Best for

PCR amplification to target specific regions of interest in conjunction with the standard library preparation for NGS


Easy-to-implement approach for smaller inputs of DNA — a cost-effective, off-the-shelf or custom genotyping method

Capture Based Panels (Hybrid-Capture)

Best for

Target enrichment using biotinylated oligonucleotide baits or probes to pull down your region of interest


Scalable to almost an unlimited number of targets per panel, enabling greater sequencing complexity and uniformity

Custom Methylation Sequencing

Best for

Specialized approach to assessing the methylation patterns using commercially available or custom hybrid capture approaches


Focus on the scientist's regions of interest, which requires less sequencing depth

16S/ITS Sequencing

Best for

Identifying the taxonomy of the bacteria and fungi population within a sample by sequencing the hypervariable regions of the prokaryotic rRNA 16S gene and ITS gene


A fast, affordable method to identify the taxonomic community of bacteria and fungi in environmental specimens

Whole Exome Sequencing

Best for

Enriching coding regions using a commercially-available targeted hybrid capture workflows.


Uniform coverage of exons that accounts for 85% of known, disease-related genetic variants

Custom Targeted Sequencing

Best for

Efficient enrichment for known, discovery-generated targets or hotspots when off-the-shelf options don’t apply.


Easy-to-use custom design options from vendors such as IDT, Agilent, and Twist Bioscience enable both amplicon sequencing and hybrid capture-based sequencing

On-Going Promotions

We're always adding new deals — check in often for updated promotions!


Targeted Sequencing Best Practices

Sample Preparation & DNA Extraction

DNA extraction from blood, cultured cells, tissue, FFPE, saliva, swab, fecal, urine, or DNA for research use

Get your high-quality genomic DNA isolated from most sample types at Psomagen.

Sample Requirements

  • 1ug of genomic DNA
  • DNA concentration ≥ 20 ng/μl
  • DIN ≥ 7.0 for non-FFPE, DIN ≥ 4.0 for FFPE

Library Preparation

  • Agilent SureSelect custom panels
  • Twist custom panel
  • IDT xGen™ Custom Hyb Panels
  • Illumina AmpliSeq



  • Illumina NovaSeq 6000
  • Illumina MiSeq

Bioinformatic Analysis

Basic analysis:

  • Data quality control
  • Mapping
  • Variant analysis (SNP/InDels)

Advanced analysis:

  • Structural variant (SV)
  • Copy number variation (CNV)
  • Annotation and statistics
  • Family trio analysis
  • Tumor-normal paired analysis
  • Custom analysis

Why Our Clients Choose Psomagen


Superior Data Quality

Exceeds manufacturer benchmarks


Fast Turnaround

Starting at one week for sequencing projects


US-Based Processing

Sequencing and customer support in Rockville, MD


Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system


Automated Workflows

Increase scalability and improve reproducibility


CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)


Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

Quotes From Our Clients

We chose you because we’ve used you before. Your services met our needs, you have good communication, and are fast responding.

Laboratory Administrator

University Research Laboratory

We chose Psomagen because of their CLIA/CAP accreditation, data quality, and price.

Postdoctoral Researcher

University Research Laboratory

Psomagen’s data quality, turnaround times, prices, and services met our needs.

Laboratory Manager

University Research Laboratory

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!


Request a Consultation

Create an account through our client portal and get in touch with your local field team to start designing a project.

We’ll help drive your research forward with the right tools for your research needs.


Place an Order

After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.

Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.


QC and Data Delivery

All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.

You can also see some project information and securely view all valid reports on the client portal site.

Targeted Sequencing in Action

Association of SNPs in the PAI1 Gene with Disease Recurrence and Clinical Outcome in Bladder Cancer
Genomic-based microsatellite development for Ternstroemia (Pentaphylacaceae) and transferability to other Ericales
Antifungal In Vitro Activity of Phoradendron sp. Extracts on Fungal Isolates from Tomato Crop
PSO Resourceful!

Want to make your targeted sequencing project more efficient and cost-effective?