Omics Research // Single-Cell/Spatial Omics
Spatial Biology
See what you discover with the ultimate systems biology tool. Uncover
mechanisms at the level of organs, tissues, cells, and sub-cellular structures.
What Is Spatial Biology?
Spatial biology is the analysis of cells within their native tissue environments.
This can be a single RNA or protein visualized on the tissue or mapping of the subcellular coordinates of every RNA transcript and protein within the individual cells that make up the tissue.
Why Is Spatial Biology Important?
Understanding the programs that are operating in each cell and being able to compare that to neighboring cells has far reaching implications for health and disease. Knowing what defines a healthy cell and tracking changes in its transcriptome and proteome while it progresses to a diseased state is leading to the development of new clinical diagnostics and therapeutics.
The newest technologies are so specific and sensitive that every single mRNA or protein can be located inside its cell within its tissue. For example, the 10X Genomics Xenium can even distinguish between different splice variants of the same mRNA.
What Can You Do?
Systems Biology
Map cell-to-cell relationships in multiple dimensions.
Disease Research
Identify novel biomarkers and potential therapeutic targets. Illuminate mechanisms of action or delivery efficiency.
Multiomics
Combine transcriptomics, proteomics, and spatial biology to generate a reference atlas for future research.
HAS ARRIVED
5,000 Genes Across Whole Tissue Sections
Our labs are now accepting orders for Xenium 5k RNA panels! These 5,000-gene panels provide transcriptomic discovery at enhanced depth and breadth. Uncover more with pan-tissue, pan-pathway assays that image thousands of cells across the full tissue section. It's a powerful tool to explore:
Pathways
Biomarkers
Ligand–receptor pairings
Cell–cell interactions
Excited to see more? You can explore a sample dataset on the 10x website.
Our Platforms
Xenium Panels with a CSP
10x Genomics Xenium panels are predesigned, validated, and curated to identify and characterize cells from human or mouse samples. Off-the-shelf panels are ready to ship. You can add up to 100 custom targets to any panel with 10x Genomics’ expert guidance.
As a 10x Genomics Certified Service Provider, Psomagen labs have the training and experience to help you explore:
Expand Spatial with CosMx
Developed by NanoString Technologies, the CosMx Spatial Molecular Imager provides multiomic insights at cellular and subcellular resolution. With exploratory capabilities and RNA and protein panels, there's no limit to what you'll discover.
Start your spatial journey at Psomagen with any of these panels:
- Human Single-Cell Spatial RNA Analysis 6,000-plex
- Human Single-Cell Spatial RNA Analysis 1,000-plex
- Immuno-Oncology Human Single-Cell Spatial RNA Analysis 100-plex
- Immuno-Oncology Human High Resolution Spatial Proteomic Analysis
- Neurology Mouse Single-Cell Spatial RNA Analysis 1,000-plex
- Neurology Mouse High Resolution Spatial Proteomic Analysis
- Mouse Spatial RNA Analysis 1,000-plex
CytAssist for Visium HD and Visium v2
Choosing an Instrument
Imaging Platforms
10x Genomics
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NanoString Technologies
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||
Xenium
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CosMx RNA
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CosMx Protein
|
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Resolution
|
Subcellular
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Subcellular
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Subcellular
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Capture Area
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10.45 x 22.5 mm
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FOVs in 15 x 20 mm
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FOVs in 15 x 20 mm
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Slides per Run
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2
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2 or 4
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4
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Organism
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Human (off the shelf)
Mouse (off the shelf) Custom (any) |
Human (off the shelf)
Mouse (off the shelf) Custom (any) |
Human (off the shelf)
Mouse (off the shelf) Custom (any) |
Tissue Type
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FFPE
FF |
FFPE
FF |
FFPE
FF |
Slide
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10x Genomics
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Leica Bond Plus
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Leica Bond Plus
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Target
|
Gene Expression
RNA |
Gene Expression
RNA |
Protein Expression
Protein |
Off the Shelf Panels
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5,000-plex RNA
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100–6,000-plex RNA
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64-plex protein
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Custom Add-ons
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Up to 100 RNA
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7–200 RNA
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Up to 8
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Stand Alone Custom Panels
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480–plex RNA
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300-plex RNA
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N/A
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Transcriptomic Platforms
10x Genomics
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Curio Trekker*
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Visium HD
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Visium CytAssist
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Slide-Seqv2
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Resolution
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2-8µm
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55µm
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10µm
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Capture Area
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6.5 x 6.5 mm
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6.5 x 6.5 mm
11 x 11 mm |
3 x 3 mm
10 x 10 mm |
Sections per Run
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2
|
2
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1
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Organism
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Human
Mouse |
Human
Mouse |
All
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Tissue Type
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FFPE
|
FFPE
FF |
FF
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Slide
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Positively charged
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Positively charged
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Curio provided
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Capture Method
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Probe-based
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Probe-based
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Oligo dT
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Sequencing Options
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Illumina
Element Ultima |
Illumina
Element Ultima |
Illumina
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Recommended Read Depth
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275M
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125M
350M |
400M
2.0B |
QC Requirements
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RIN >7
DV200>30% |
RIN>7
DV200>30% |
RIN>7
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*Coming soon to Psomagen labs.
Need Help Choosing Your Spatial Platform?
Spatial Biology Best Practices
Sample Types
A wide variety of sample types are suitable for spatial analysis. Whether starting from fresh frozen tissue in a tube or formalin-fixed paraffin-embedded sections dried onto Xenium slides, high quality spatial data is achievable.
Data Delivery
Standard report includes H&E QC images, decoded data containing cell clusters, transcripts per cell, and differential gene expression.
We're Ready for Your Order!
Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!
01
Request a Consultation
Create an account through our client portal and get in touch with your local field team to start designing a project.
We’ll help drive your research forward with the right tools for your research needs.
02
Place an Order
After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.
Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.
03
QC and Data Delivery
All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.
You can also see some project information and securely view all valid reports on the client portal site.
New to Spatial Biology?
Experimental design in a new area like spatial biology can be challenging. Take advantage of expert resources to plan for success.
- Consortia including the Human BioMolecular Atlas Program (HuBMAP) and the Human Cell Atlas (HCA)
- Nature's series on HuBMAP's production of reference maps
- Xenium best practices
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Third Generation Sequencing Is Here!
See how the newest technologies are providing deeper insights in omics research.
Omics Insights Blog
November 20, 2024
November 1, 2024